Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetMeasuring Anthrax lethal factor (LF) metalloproteolytic activity

General description

An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax lethal factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors.

An N-acetylated, C-7-amido-4-methylcoumarin (AMC) derivative of a 14-mer peptide substrate designed from the MEK-2 template that is useful for measuring Anthrax Lethal Factor (LF) metalloproteolytic activity with a detection limit of ~5-10 pM. Reported to be useful for high-throughput screening of LF inhibitors. Excitation max.: ~360 nm; emission max.: ~460 nm.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Tonello, F., et. al. 2002. Nature418, 386.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Ac-Gly-Tyr-βAla-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Val-Leu-Arg-AMC

Warning

Toxicity: Standard Handling (A)